Nicotine Test COT – reliable rapid test for detecting nicotine exposure

✅ Accurate and fast nicotine screening – no lab required

The COT nicotine test is a urine-based rapid test that detects cotinine, the primary metabolite of nicotine. It provides results within minutes and is CE-marked for professional use. Due to its ease of use, it’s also suitable for individuals monitoring their own status.

This test detects nicotine exposure from all sources (smoking, vaping, nicotine gum, patches, sprays) up to 2–3 days after use. It’s a valuable tool for verifying abstinence, supporting cessation programs, or performing workplace or clinical screenings.

Nicotine is a toxic alkaloid that affects the nervous system. Even non-smokers can be exposed through passive smoking. Cotinine is more stable and longer-lasting than nicotine itself, which makes it a more reliable biomarker in urine.

🔬 How it works

Nicotine quickly metabolizes into cotinine
Cotinine remains detectable in urine for up to 72 hours
Urine pH does not significantly affect the result
More reliable than testing for nicotine directly

👥 Who is this test for?

Healthcare professionals in addiction care, occupational health, school health
Youth programs, family care, and residential services
Private users monitoring their own progress

🚬 Detects all nicotine sources

Cigarettes
Snus
Vape and e-cigarettes
Nicotine gum, patches, sprays

⚙️ Technical details

Sample type: urine
Sensitivity: 10 ng/ml
Result time: 3–8 minutes
Accuracy: 99.8% vs laboratory testing
CE-marked for professional IVD use

📦 Package includes

1 nicotine test strip (COT)
English instructions for use
Requires a clean sample cup and a timer (not included)

🧾 Instructions – COT Nicotine Test

1. Collect a urine sample in a clean container. The sample can be taken at any time of day.

2. Allow the sample to reach room temperature if it has been refrigerated or frozen.

3. Open the test strip pouch and remove the strip.

4. Dip the strip into the urine up to 1 cm (not over the MAX line). Hold for 10–15 seconds.

5. Place the strip horizontally on a flat surface. Wait exactly 5 minutes.

6. Read the result:

🟢 Negative: Two lines (C + T) = no nicotine above the cutoff level
🔴 Positive: Only the C line = nicotine detected
⚠️ Invalid: No C line = test failed, repeat with a new strip