A vitamin D rapid test is performed on a fingerstick blood sample. The sensitivity of the test is 30 ng/ml ± 4 ng/ml.
Quality tests have found that the accuracy of this rapid test was more than 99.9% in indicating vitamin deficiency, 94.1% in indicating insufficient vitamin intake and 92% in demonstrating adequate vitamin intake. The consistency of inter and intratests with different vitamin D levels is more than 99%. The test is not interfered with by other vitamins (A, B, C, E, K, M).
The test kit contains individually packed test cartridges, 2 lancets, disposable capillary pipettes, one buffer liquor bottle, a colour chart for interpreting the result and instructions in English.
Vitamin D Rapid Test Instruction Manual
- Start testing only when all necessary reagents and equipment are at room temperature (+ 15-30 °C).
- Take the test cartridge out of its foil bag and lay it down on the table
- Take a fingerstick blood sample from a clean and dry area after washing with soap and warm water or disinfecting with alcohol. Blood circulation in the fingertips is improved by hand washing with hot water. Using a lancet, poke a hole in the side of the middle finger or ring finger and wipe off the first drop of blood with soft paper. If necessary, rub the palm of the hand and the base of the finger towards the tip of the finger to reveal a round but rather large drop of blood.
- Touch a drop of blood over the capillary and allow the blood to absorb and fill the capillary (approx. 20 μl). Avoid the formation of air bubbles. Completely empty the capillary into the S-well of the test cartridge by squeezing the upper end of the capillary pipette. Add two drops of bumper from the buffer bottle to the S-pit and start the timer or check the time on the clock.
- Let the colored liquid front calmly advance over the cassette observation window, waiting until the background lightens and reddish-brown lines appear.
- To see the result 10 minutes later (no more than 20 minutes later) after adding the sample to the cassette, compare the intensity of the color of the T-line with the color chart and interpret the result as follows:
- Vitamin D deficiency is evident at concentrations up to 10 ng/ml.
- Vitamin D intake is insufficient at concentrations of 10-30 ng/ml.
- Vitamin D intake is sufficient at concentrations of 30-100 ng/ml.
- The result is always void and testing must be repeated when the C-line is missing.
Vitamin D
Vitamin D is a group of fat-soluble steroid derivatives that increase the absorption of calcium, iron, magnesium, phosphate and zinc in the intestine. In humans, the most effective of these compounds are vitamins D3 and D2. Of these, D3 is formed in the skin catalyzed by UV radiation from sunlight, whereas D2 mainly comes from food. The liver converts Vitamins D into 25-hydroxyderivatives, the determination of which from the bloodstream describes the level of vitamin D in the body.
Vitamin D deficiency
Vitamin D deficiency is now considered a global epidemic. Almost all human cells contain vitamin D receptors, meaning they need sufficient vitamin D levels to function normally. Vitamin D deficiency has been shown to be linked to a number of diseases, such as: rickets, osteoporosis, osteomalacia, multiple sclerosis, cardiovascular diseases, pregnancy complications, diabetes, depression, strokes, autoimmune diseases, flu, cancers, infectious diseases, Alzheimer’s, obesity and abnormally high mortality. Monitoring of vitamin D levels is currently considered medically necessary to ensure adequate vitamin D levels in order to avoid diseases caused by deficiency and improve general health.
If your vitamin D level is too low or deficient, consider taking a daily vitamin D supplement as recommended. It is important to follow the advice given by your healthcare professional regarding the appropriate daily dose of vitamin D.